Figure 5.

Time Course for the Phosphorylation of WT and p.Val665Ala PDGFRB Receptors

Representative western blots of transfected HeLa cells treated with PDGF-BB at 5, 15, 30, and 60 min and blotted to detect total PDGFRB (A), phosphorylated PDGFRB at tyrosine 751 (p-Tyr751) (B), and tyrosine 1021 (p-Tyr1021) (C). Horizontal bars indicate the electrophoretic mobility of the two main PDGFRB forms (180 and 160 kDa) (D). Schematic representation of the quantitative data obtained by western blot of the time-dependent change in the PDGF-BB-induced phosphorylation of PDGFRB (n = 3). Data are expressed as the fold change (average ± SEM) in p-Tyr751 and p-Tyr1021 immunoreactivity at 180 and 160 kDa bands compared to the corresponding band of total PDGFRB. ^∗ = p < 0.05, ^∗∗ = p < 0.01, ^∗∗∗ = p < 0.001, ^∗∗∗∗ = p < 0.0001.