Figure 6.

Time Course for the Phosphorylation of PDGFRB Downstream Signaling Molecules in HeLa Cells Transfected with WT and p.Val665Ala Constructs

Representative western blots of transfected HeLa cells treated with PDGF-BB at 5, 15, 30 and 60 min blotted to detect phosphorylated AKT (pAKT) and total AKT (A), phosphorylated STAT3 (pSTAT3) and total STAT3 (B), phosphorylated PLCγ1 (pPLCγ1) and total PLCγ1 (C), phosphorylated SRC (pSRC) and total SRC (D), and phosphorylated ERK (pERK) and total ERK (E). α-tubulin is shown as a protein loading control (F). Molecular size is indicated in kDa. A schematic representation of the quantitative data obtained by western blot of the time-dependent change in the PDGF-BB-induced phosphorylation of AKT, STAT3, PLCγ1, SRC, and ERK is shown at the right of the corresponding blots (n = 3). Data are expressed as the fold change (average ± SEM) in pAKT, pSTAT3, pPLCγ1, pSRC, and pERK in comparison to total AKT, STAT3, PLCγ, SRC, and ERK, respectively. ^∗ = p < 0.05, ^∗∗ = p < 0.01.