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Low-resolution structural analysis of IscU by SAXS. (A) D-state Rg values calculated from experimental SAXS data using Eq. 1 (black) and from disordered structures selected from a two-state MES (red). (B) Rg values for members of the ensemble of structures used for the MES analysis. (C) Fractional S-state population derived from the conformers selected by the two-state MES fit (red) compared with that determined by NMR (black).
