Fig. 6.

LRIM9 does not interact with the known complement-like system. a Hemolymph was collected from dsGFP-, dsLRIM1- and dsLRIM9-injected mosquitoes 24 h after an uninfected murine blood meal. Samples were analyzed by nonreducing Western blot using antibodies against TEP1, SRPN3, LRIM9 and APL1C (for LRIM1/APL1C). b Conditioned medium was collected 3.5 days after transfection of Sua4.0 cells with secreted His-tagged GFP (sGFP), LRIM9 and APL1C. Tagged proteins and interacting partners were captured from the conditioned medium using metal affinity beads. Starting conditioned medium (left panels) and bound material (right panels) were analyzed by nonreducing Western blot. Two blots were probed with a His probe (top panels) and a TEP1 antibody (bottom panels), respectively.