Table 1.

LRIM9 silencing has no effect on egg laying and larval hatching

Gene knockdown	Females at start	Females laid eggs	Total eggs laid	Mean eggs per female	Females laid eggs	Hatchability	Fertile Females
GFP	46	27	1,809	67	59%	62%	50%
LRIM9	46	29	1,958	68	63%	64%	52%

Mosquitoes were allowed to mate, treated with dsGFP and dsLRIM9 and then blood fed. After 72 h, individual females were placed in dishes with wet filter paper and encouraged to lay eggs in darkness. The number of eggs laid and larvae hatched were counted. Hatchability is the percentage of eggs that hatched into larvae. Fertile females were defined as those that lay at least one egg and produce at least one larva.