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. 2015 Sep 3;97(3):445–456. doi: 10.1016/j.ajhg.2015.08.002

Figure 5.

Figure 5

Knockdown of ARID1B in Neuro2A Cells Leads to Neurite Outgrowth through β-Catenin

(A) Western blotting (WB) for ARID1B, β-catenin and β-actin from lysates of mouse neuroblastoma Neuro2A cells transfected with siRNAs against control, ARID1B or ARID1B, and β-catenin, as indicated. Molecular weights (kDa) are indicated.

(B) Ratio of TOP/FOPFlash luciferase activity measuring β-catenin-dependent transcription in Neuro2A cells transfected with indicated siRNAs.

(C) Representative immunofluorescence pictures of Neuro2A cells transfected with siRNAs against control or ARID1B and β-catenin as indicated, stained for α-tubulin. Arrowheads point to neurites.

(D) Quantification of cells from (C) indicating the percentage of cells exhibiting neurites with twice the length of the cell body. n = number of cells analyzed from three independent experiments. Error bars in (B) and (D) represent SEM from two and three independent experiments, respectively. Asterisks denote statistical significance (p value of <0.05) as compared to respective controls indicated in the main text, calculated with unpaired Student’s t test.