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. 2015 Jul 8;54(35):5447–5456. doi: 10.1021/acs.biochem.5b00374

Table 3. Solvent Isotope Effect at 30 °Ca.

enzyme form D2Okcat D2O(kcat/Km)
WT SLO-1 1.20 (0.10) 1.52 (0.31)
553A/754A 1.46 (0.11) 1.27 (0.27)
546A/754A 1.04 (0.12) 0.91 (0.28)
a

H2O buffer: 0.1 M borate (pH 9.0) in H2O. D2O buffer: 0.1 M borate (pD 9.0) in D2O (with a pH meter reading of 8.6).