Skip to main content
. Author manuscript; available in PMC: 2015 Sep 10.
Published in final edited form as: Mol Carcinog. 2013 Oct 22;53(0 1):E169–E180. doi: 10.1002/mc.22088

Figure 1.

Figure 1

Reg4 accelerates G1/S-G2/M phase transition and increases mitotic index of human CRC cells. Propidium iodide-based flow cytometric analyses were performed to determine the effect of Reg4 on CRC cell population in different phases of cell cycle. Addition of rhR4 (200 nM; 24 h) to HCT116 cells’ media significantly decreased cell number in G1 phase and increased in G2/M phase (upper panels). Lower left panel shows the quantitative analysis of the above-mentioned study. Following colorimetric mitotic assays, rhR4 (200 nM; 24 h) treatment significantly increased the number of HCT116 cells undergoing mitosis as shown by an increased mitotic index (lower right panel); *P < 0.05, **P < 0.01.