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. Author manuscript; available in PMC: 2015 Sep 10.
Published in final edited form as: Mol Carcinog. 2013 Oct 22;53(0 1):E169–E180. doi: 10.1002/mc.22088

Figure 4.

Figure 4

Reg4 induces expression of Cyclin D1, Cyclin D3, CDK4, and CDK6 in human CRC cells. An addition of rhR4 (200 nM) to HCT116 and HT29 cells led to a time-dependent increase in mRNA (panel A) and protein (panel B) expression of Cyclin D1 and Cyclin D3. No change in Cyclin E1 mRNA and protein expression shown in corresponding panels is used as a negative control. In addition, Reg4 treatments led to time-dependent increase in protein expression of CDK4 and 6 (panel C). No change in CDK2 protein expression shown in this panel is used as a negative control; *P < 0.05, **P < 0.01.