Skip to main content
. Author manuscript; available in PMC: 2015 Sep 10.
Published in final edited form as: Mol Carcinog. 2013 Oct 22;53(0 1):E169–E180. doi: 10.1002/mc.22088

Figure 6.

Figure 6

Antagonism of Reg4-signaling inhibits human CRC cell proliferation. Reg4-specific function blocking mAbs (3E5 and 2H6) and Akt inhibitor were used to antagonize Reg4 signaling and tested their effects on human CRC cell proliferation by performing MTT (A) and EdU flow cytometry (B) assays. Treatments of Reg4-specific mAbs (3E5 and 2H6: 100 μg/mL; 24 h) and Akt inhibitor (10 μM; 24 h) led to significant decreases in proliferation rate of HT29 cells. Treatments with rhR4 (200 nM; 24 h) and SB216763 (10 μM; 24 h) positively increased the proliferation rate. However, the addition of rhR4 to HT29 cells pretreated 1 h before with Akt inhibitor (10 μM) did not increase CRC cell proliferation. Results identify Reg4 as a potent regulator of human CRC cell proliferation; *P < 0.05, **P < 0.01.