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Fluorescent-ligand binding to the A2AR variants. HEK-293 cells transiently transfected with untagged A2AR constructs and incubated with 70 nM FITC-APEC. A–C) Variants with mutations in the cysteines in ECL1. D–F) Variants with mutations in the cysteines in ECL2. G–I) Variants with mutations in the cysteines in ECL1 and ECL2, corresponding to the disulfide bonds. J–K) WT A2AR and negative control. Scale bars, 10 µm.
