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. 2015 Jul 15;8(7):10481–10490.

Figure 4.

Figure 4

DUSP6 is a direct target of miR-145 and miR-145 inhibited the growth of MDA-MB-231 cells. A. The binding site for miR-145 in the 3’-UTR of DUSP6 mRNA; B. Western blot analysis was used to detect DUSP6 protein expression levels; C. The relative luciferase activity (Renilla/firefly) was measured in HEK293T cells after co-transfection of the DUSP6 3’-UTR or DUSP6 3’-UTR mutant luciferase construct with either miR-145 mimics or miR-NC; D-F. MTT and Colony formation assays showed Crystal Violet staining of the miR-145-transfected group and NC-transfected group (Comparison was draw by the t-test (two-tailed). Data represent means ± SD; *P<0.05).