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PCR reaction system and amplification temperature cycle parameters
| Components | Volume per reaction |
|---|---|
| SsoAdvanced SYBR Green Super mix | 5 μL |
| Forward primer (10 μM) | 0.3 μL (300 nM) |
| Reverse primer (10 μM) | 0.3 μL (300 nM) |
| cDNA template | 100 ng |
| Nuclease-free water | Up to 10 μL |
