Table 2.
Characteristics and polymorphism revealed by ISSR primers for 95 wheat genotypes used in the study.
| ISSR primer | Sequence | Melting temperature (Tm) | Total number of bands | Polymorphic bands | Per cent polymorphism detected |
|---|---|---|---|---|---|
| ISSR F3 | 5′-(AG)8 CG-3′ | 56.0 | 8 | 7 | 87.5 |
| ISSR F4 | 5′-(AG)8 TG-3′ | 53.7 | 12 | 12 | 100 |
| ISSR F9 | 5′-(GAA)5-3′ | 39.6 | 13 | 12 | 92.3 |
| ISSR M1 | 5′-(AGC)6 G-3′ | 63.1 | 12 | 11 | 91.6 |
| ISSR M2 | 5′-(ACC)6 G-3′ | 63.1 | 14 | 14 | 100 |
| ISSR M3 | 5′-(AGC)6 C-3′ | 63.1 | 17 | 16 | 94.1 |
| ISSR M8 | 5′-(AC)9 G-3′ | 56.7 | 13 | 12 | 92.3 |
| ISSR M9 | 5′-(AC)8 CG-3′ | 56.0 | 13 | 13 | 100 |
| ISSR M12 | 5′-(GACAC)4-3′ | 61.4 | 6 | 6 | 100 |
| ISSR M17 | 5′-CAG (CA)8-3′ | 56.7 | 8 | 7 | 87.5 |
| Total | 116 | 110 | 94.8 |