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Experimental design of the EVTs culture. EVTs were cultured under conditions of 2% O2 for 24 hours to promote attachment to the Matrigel. The cells were then cultured for an additional 24 hours under conditions of 2% O2 with or without 200 μmol/L of vitamin C or 8% O2 with or without 200 μmol/L of vitamin C. The expression levels of genes related to mitochondria-dependent apoptosis and ROS were subsequently analyzed. EVT indicates extravillous trophoblast.
