Figure 3.

Expression of IL1B, CD14, NFKB1, and RIPK1 measured by quantitative and digital PCR. Expression of the identified proinflammatory genes was measured in a larger cohort; women with chorioamnionitis (at consent [n = 10] and at delivery [n = 19]), PPROM no chorioamnionitis (at consent [n = 5] and at delivery [n = 8]), and gestational-matched controls (n = 12) by quantitative PCR (A, C, E, and G) and digital PCR (B, D, F, and H). Mean and SEM are graphed, *P < .05. IL1B indicates interleukin 1β; NFKB1, nuclear factor-κB1; RIPK1, TNF receptor super family-interacting serine–threonine kinase 1; PCR, polymerase chain reaction; PPROM, preterm prelabor rupture of the fetal membranes; SEM, standard error of the mean.