Fig 7. Effects of different caspofungin concentrations on MAPK cascades.
(A) When caspofungin is used at inhibitory concentrations (1 μg ml-1), a repression of MpkA and SakA phosphorylation levels is observed. Under these conditions, the cellular transport is stimulated and the inhibition of MpkA and SakA avoids the turn-on of compensatory pathways. (B) The use of caspofungin at higher doses strongly activates MpkA and SakA. Under these conditions, cellular transport is inhibited and cell-wall compensatory pathways are more active.