Table 2. The results of miRNA RT-qPCR with a series of diluted RNA templates.
| Total RNA | Cт | ΔCт | +/- | |||
|---|---|---|---|---|---|---|
| (ng) | miR10b | miR135b | RNU6b | [10b-U6] | [135b-U6] | |
| 100 | 27.392 | 26.29 | 35.942 | -8.55 | -9.652 | + |
| 10 | 30.327 | 29.68 | 38.968 | -8.641 | -9.288 | + |
| 1 | 31.788 | 33.449 | undetermined | —— | —— | Failed* |
| 0.1 | 32.199 | 36.752 | undetermined | —— | —— | Failed |
| 0.01 | 31.938 | 49.227 | undetermined | —— | —— | Failed |
| 0.001 | 32.381 | undetermined | undetermined | —— | —— | Failed |
| NTC** | undetermined | undetermined | undetermined | —— | —— | Failed |
Note *: Failed: When test results of reference gene is undetermined, it is considered that RNA extraction is unsuccessful or the concentration of RNA is too low to be amplified.
** NTC: negative control.