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. 2015 Sep 10;10(9):e0136778. doi: 10.1371/journal.pone.0136778

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© 2015 Popova et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Gel-eluted fragments from C-fragment libraries after restriction digestion (10% PAGE). The randomized fragments are marked in red; the brackets indicate the C-fragments, participating in one ligation assembly. Marker: 50 bp ladder (Thermo Scientific) (B) Ligation assembly of B1-fragment prior to gel elution. The full-length ligation product is indicated with an arrow. The length of the assembled B1 fragment with flanking BsaI restriction sites comprises 208 bp. Marker: 50 bp ladder (Thermo Scientific) (C) Gel-eluted fragments from B-fragment libraries. The length of the B1 fragment, liberated from the plasmid vector after restriction digestion comprises 182 bp. (D) Gene library ligation assembly. The full-length ligation product is indicated with an arrow. Marker: 1 kb ladder (Thermo Scientific).