Table 5. Summary of genetic complementation results.
| Clone | A8 | A54 | S55 | H84 | A128 | A204 | L222 | A223 | S225 | Doubling time (min.) | Standard dev. (min.) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | 152 | 8 | |||||||||
| F1 | R | G | T | 126 | 5 | ||||||
| F2 | R | 147 | 11 | ||||||||
| F3 | N | T | 150 | 4 | |||||||
| F4 | G | N | 157 | 4 | |||||||
| F5 | T | 160 | 10 | ||||||||
| F6 | G | 161 | 6 | ||||||||
| F7 | Δ3 | T | 161 | 2 | |||||||
| F8 | N | 162 | 9 | ||||||||
| F9 | Δ3 | V | T | 172 | 11 | ||||||
| F10 | T | 206 | 6 | ||||||||
| F11 | T | 143 | 2 |
Amino acid substitutions in library members that complemented the HisF deficiency. The growth rate of ΔhisF cells, transformed with the corresponding plasmid variants was determined. The doubling time is a mean of five independent experiments.