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. 2015 Sep 10;10(9):e0137255. doi: 10.1371/journal.pone.0137255

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© 2015 Okolicsanyi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — ICC showing positive staining for each of the five markers used to confirm hMSC stemness. Each of three hMSC populations was stained for each marker at each phase of growth. Cells are positive for: A-C: goat anti-mouse-CD44 (1/1000); D-F: goat anti-rabbit-CD29 (1/200); G-I: goat anti-mouse-CD90 (1/500); J-L: goat anti-mouse-CD105 (1/500); and negative for M-O: goat anti-rabbit-CD45 (1/500) at Phase A, Phase B and Phase C. At Phase C populations also become positive for CD45. Secondary antibodies used were anti-mouse-Chromeo546 (yellow; 1/1000) and anti-rabbit-Chromeo488 (green; 1/1000). Cells were mounted in mounting media containing DAPI (blue) to counterstain nuclei. Scale bar represents 70μm.