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. 2015 Sep 10;10(9):e0137255. doi: 10.1371/journal.pone.0137255

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© 2015 Okolicsanyi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Relative gene expression changes for A: Stemness markers (CD44); lineage specific B-E: adipogenic (C/EBPa, C/EBPd, PPARG1/2); F-H: Fibroblastic (ACTA2, FN1, FSP1); and I-L: osteogenic (AP, COL1A1, BSPII, OCN) in hMSCs (n = 3) at each passage. The majority of genes examined (lineage and stemness) increased expression at Phase B followed by decreased expression by Phase C (ACTA2, CD44, C/EBPd, COL1A1, FN1, FSP1). In contrast, lineage specific markers osteocalcin; OCN, Alkaline Phosphatase; AP and PPARG1 demonstrated decreased expression throughout expansion with bone sialoprotein II; BSPII, C/EBPa and PPARG2 demonstrated increased expression throughout expansion.