Table 1. Primary and secondary antibody concentrations for; Immunocytochemistry (ICC) and Fluorescence Assisted Cell Sorting (FACS).
| Primary Antibody | Dilution | Application | Secondary Antibody | Dilution |
|---|---|---|---|---|
| CD29 | 1/100 | ICC, FACS | Chromeo488 | 1/1000 |
| CD44 | 1/500 | ICC, FACS | Chromeo546 | 1/1000 |
| CD45 | 1/500 | ICC, FACS | Chromeo488 | 1/1000 |
| CD90 | 1/200 | ICC | Chromeo546 | 1/1000 |
| CD105 | 1/200 | ICC | Chromeo546 | 1/1000 |
| Nestin | 1/200 | ICC, FACS | FITC | 1/1000 |
| Sox2 | 1/1000 | ICC, FACS, | Cy3 | 1/1000 |
| GFAP | 1/500 | ICC | Cy3 | 1/1000 |
| MAP2 | 1/200 | ICC | FITC | 1/1000 |
| O1 | 1/500 | ICC | AlexaFluor594 | 1/500 |
| Mouse IgG | 1/1000 | ICC | FITC/Chromeo546 | 1/1000 |
| Mouse IgM | 1/500 | ICC | AlexaFluor594 | 1/500 |
| Rabbit IgG | 1/1000 | ICC | Cy3/Chromeo488 | 1/1000 |