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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Mol Immunol. 2015 Jun 20;67(2 0 0):265–275. doi: 10.1016/j.molimm.2015.06.001

Figure 1. Cell purity and co-localization of FABP5 expression with expression of the hepatic macrophage marker F4/80.

Figure 1

A: Cell colonies were obtained from the livers of C57Bl/6 mice as described in materials in methods. The mRNAs of hepatocyte (HC) marker albumin, hepatic Kupffer cell (KC) marker F4/80, hepatic stellate cell (HSC) marker α -SMA, T-cell (T) marker CD3, endothelial cell (EC) marker CD31, and FABP5 were detected by reverse transcription polymerase chain reaction. B: Immunohistochemistry was performed on sections of liver from C57Bl/6 (WT) mice treated with saline as control (0 h) or LPS for 24 hours. Antibodies to the hepatic macrophage marker F4/80 and FABP5 were used. Merged image shows colocalization of F4/80 and FABP5 expression. Micrographs are at 40x magnification. Arrows point to hepatic macrophages.