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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Mol Immunol. 2015 Jun 20;67(2 0 0):265–275. doi: 10.1016/j.molimm.2015.06.001

Figure 2. LPS promotes hepatic FABP5 expression.

Figure 2

Wildtype and FABP5−/− mice were treated with saline (control) or LPS for 3, 9 or 24 hours. A: Control whole liver FABP5 mRNA expression was analyzed via quantitative PCR and normalized to the housekeeping gene 18S rRNA. B: WT liver macrophages were isolated as stated earlier and FABP5 mRNA expression was analyzed via quantitative qPCR and normalized to 18s rRNA. C: Immunohistochemistry was performed on sections of liver tissue from WT mice treated with LPS for 0, 9, or 24 hours using an antibody to FABP5. D: Percent stained area was determined by image J analysis in three separate measurements from each time point.