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. Author manuscript; available in PMC: 2016 Mar 7.
Published in final edited form as: Phys Rev E Stat Nonlin Soft Matter Phys. 2015 Apr 7;91(4):042703. doi: 10.1103/PhysRevE.91.042703

Figure 4.

Figure 4

Volumetric imaging using 2P-SPIM. (a) Two-dimensional 2P-SPIM reconstructed image of ganglion cell dendrites in ground squirrel retina at a depth of 100 μm from the vitreal surface. A magnified comparison (within the yellow box) of (b) direct 2P microscopy (grayscale) and (c) 2P-SPIM (pseudo-colored) images. The 2P-SPIM reconstruction can resolve the object with a size of ~145 nm, which is still much beyond its diffraction limit, at the depth of 100 μm. Three-dimensional image stacks at depths from 94 μm to 110 μm in the retinal tissue were created using both (d) direct 2P microscopy (grayscale) and (e) 2P-SPIM (pseudo-colored). A total of 16 slices acquired at 1 μm depth interval were stacked to form the volumetric images. The cell soma, at top, is removed. Z-axis resolution is limited by the 2P point spread function.