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. 2015 Aug 28;6(10):1020–1029. doi: 10.7150/jca.12733

Fig 2.

Fig 2

Effect of 10 or 100 μM zoledronate treatment on gelatinase (MMP-2 and -9) expressions and activities. A) Gelatinase expressions and activities by zymography at day 2 or 3 and representative zymogram. Relative proteinase activities of pro and active MMP-9 and active MMP-2 were expressed as mean ± S.E.M. (n=4) * P ≤ 0.05, C versus Z100; + P ≤ 0.05, ++ P ≤ 0.01, Z10 versus Z100; Paired Student's t-test. B) MMP-9 expression on western blotting and a representative western blot. Relative expression of MMP-9 was expressed as mean ± S.E.M. (n=4)** P ≤ 0.01 C versus Z100; ++ P ≤ 0.01 Z10 versus Z100; Paired Student's t-test. C) Immunopositivity scores of both gelatinases and representative photomicrographs of PC3 cells stained with MMP-2 or MMP-9 antibodies immunocytochemically at day 3. Immunpositivity scores were expressed as mean ± S.E.M. (n=4) * P ≤ 0.05, ** P ≤ 0.01, C versus Z100 Chi-square test. C: Control, Z10: 10 μM zoledronate, Z100: 100 μM zoledronate. C: Control, Z10: 10 μM zoledronate, Z100: 100 μM zoledronate.