Fig 4.

Effects of 10 or 100 μM zoledronate treatment on protein and mRNA expressions of TIP. A) TIP expression on western blot at day 2 or 3 and representative western blots. Relative expression of TIP were expressed as mean ± S.E.M. (n=4) * P ≤ 0.05 C versus Z100; ++ P ≤ 0.01, Z10 versus Z100; Paired Student's t-test. B) Immunopositivity scores of TIP and representative photomicrographs of PC3 cells stained with TIP antibody. Immunpositivity scores were expressed as mean ± S.E.M. (n=4) ** P ≤ 0.01 C versus Z100 Chi-square test. C) TIP mRNA expressions at day 3. mRNA fold changes were expressed as mean ± S.E.M. (n=4) ** P ≤ 0.01 C versus Z100, Paired Student's t-test. TIP: Type I procollagen, C: Control, Z10: 10 μM zoledronate, Z100: 100 μM zoledronate.