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The knockdown of NUMB increased the proliferation of Ishikawa cell. 3A. shows the success of siNUMB method tested by q-PCR. 3B. shows the success of siNUMB method tested by WB. 3C. shows the increased proliferation of Ishikawa cell with knock down of NUMB tested by CCK8 assay. 3D. shows the bar graph of PCNA assay through QPCR method. 3E. shows the proliferation index by determined cell cycle with flow cytometer assay.
