Fig. 10.

Secondary structure of IFNA5 RNA (197–446) wt and Mut-1 to Mut-4 summarizing enzymatic probing. Upper draw secondary structure of IFNA5 RNA (197–446) recompiling the location of the site-directed mutational changes in the individually analysed mutant sequences. The yellow squares indicate mutations of Mut-1 to Mut-4 sequences. The blue circles are mutations in PK1 and PK2 and the compensatory Mut-PK1+2 sequences. The nucleotide changes are provided above the wild-type sequence. Results from RNase T1 and RNase V1 partial digestion data from 5′ end-labelled IFNA5 RNA (197–446) are represented by arrowheads and circles, respectively. Mut-1 to Mut-4 are depicted. Only new cleavage points or missing cleavages specific for each of the single mutant sequences in relation to wt are presented. Key changes in boxes. The results shown are from analysing 10 % as well as 6 % denaturing polyacrylamide gels