Fig. 6.

Enzymatic probing of the secondary structure of IFNA5 RNA (197–446). a, b 5′-[³²P] end-labelled RNA. b, c 3′-[³²P] end-labelled RNA. For a and b lane 1 is the RNA maintained on ice (I); lane 2 alkaline hydrolysis reaction (OH); lane 3 RNase T1 reaction under denaturing conditions (T1L); lane 4 RNase T1 (T1), lane 5 RNase V1 (V1) and lane 6 RNase A (A) under standard conditions, respectively. For c and d products digested with alkali (OH), RNase T1 under denaturing conditions (T1L), RNase T1 (T1), RNase V1 (V1), RNase A (A) and E. coli RNase 1 (1) under standard conditions were analysed in lanes 1 to 6, respectively. Denaturing gels were at 10 (a), 6 (b and d) and 15 % (c) polyacrylamide. The numbers on the right indicate the point of digestion cleaved by RNase T1 under denaturing conditions (T1L), as identified with the help of the OH sequence ladders run on the left