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. 2015 Apr 22;72(19):3747–3768. doi: 10.1007/s00018-015-1908-0

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — Chemical probing of 3′ end-labelled IFNA5 RNA (197–446). a, b DEPC probing. Lane 1 is the RNA maintained on ice (I). Lanes 2 and 3 are the products from digestion with alkali (OH) and RNase T1 under denaturing conditions (T1L), respectively. Products of aniline-treated RNAs previously modified with DEPC under native conditions (lane 4), semi-denaturing conditions (lane 5) and denaturing conditions (lane 6). c, d Pb²⁺ probing. Lane 1 is the RNA maintained on ice (I). Lane 2 treatment with alkali (OH); lane 3 treatment with RNase T1 under denaturing conditions (T1L); lane 4 treatment with Pb²⁺ at 4ºC for 15 min and lane 5 treatment with Pb²⁺ at 30ºC for 20 s. Denaturing gels were at 10 % (a, c) or 6 % polyacrylamide (b, d)