Fig. 9.

Regions of IFNA5 RNA subdomains protected from RNase T1 digestion under native conditions. a Protection of IFNA5 RNAs from RNase T1 digestion assay. Internally labelled IFNA5 RNAs (197–446), (215–427) and (329–427) maintained on ice (lanes 1–3), incubated with digestion buffer (lanes 4–6), or in the presence of RNase T1 at saturated concentration (lanes 7–9). Prominent product species like those indicated by arrows were eluted for further study (supplementary Fig. 14). Denaturing gel was at 15 % polyacrylamide. b Region protected from RNAse T1 digestion bases written in bold are more protected from digestion with RNase T1 than the ones in standard style. Numbers indicate the nucleotide positions