Figure 4. Crystal structure of the E. cuniculi Mot1^NTD:TBP:DNA:NC2 complex.

Front and side views of the structure, represented as cartoon models with highlighted secondary structure. TBP (blue), NC2α (light green), and NC2β (dark green) encircle kinked promoter DNA (gray with upstream (U) and downstream (D) regions labeled). The HEAT repeats (HR, yellow with N- and C-termini marked) and insertion domain (orange) of the Mot1^NTD bind along the convex surface of TBP and contact the C-terminal helix (H5) of NC2β. The latch of Mot1^NTD (magenta), which has been previously shown to bind to TBP's DNA-binding cleft, is mostly disordered in the presence of DNA and NC2.

DOI: http://dx.doi.org/10.7554/eLife.07432.008

Figure 4—figure supplement 1. X-ray electron density maps.

(A) 2F_o – F_c electron density map of DNA bases displayed at 1σ contour level (gray mesh). TBP is shown in blue. The widening at the TATA box is nicely visible. (B) Anomalous density map (blue mesh) contoured at 3σ level shows signal of some of the selenium sites of NC2α (left), NC2β residues 12–101 (middle) and NC2β residues 110–137 (right).

DOI: http://dx.doi.org/10.7554/eLife.07432.009