Reactivity profiles of mAb IPS-K-4A2B8 on solid tumor cell lines. Cells were labeled with mAb IPS-K-4A2B8 using indirect immunofluorescence staining as described in Methods. Cells were analyzed on a FACSCanto flow cytometer. Data were processed using the FCS Express software. Note that antibody IPS-K-4A2B8 reacts with most cells of embryonal carcinoma-derived cell lines (NT2, NCCIT, 2102Ep) and only with a subpopulation of cells in many other cancer cell lines (HCT-116, Caco-2, MCF-7, SW480, SW620, PC3, DU145, TE671, TCam-2, MG-63).