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. 2015 May 15;25(8):902–917. doi: 10.1093/glycob/cwv032

Fig. 6.

Fig. 6.

Localization of SSEA-4 and organelle-specific markers on PC3 cells. Adherent PC3 cells were stained for organelle-specific markers including caveolin-1 (caveolae), GM130 (Golgi apparatus), Rab11 (recycling endosomes), EEA1 (early endosomes), Lamp2 (lysosomes) or cortactin (invadopodia) and SSEA-4. SSEA-4 accumulating structures showed no colocalization with caveolin-1, GM130, Rab11, EEA1 and Lamp2, suggesting that SSEA-4 accumulating puncta does not correspond to caveolae, Golgi apparatus, recycling endosomes, early endosomes and lysosomes. On the other hand, SSEA-4 strongly colocalized with cortactin (marked by up-arrow), indicating that SSEA-4 accumulating puncta correspond to invadopodia. Scale bars: 10 µm.