Fig. 3.

Effects of wogonin on IL-4-induced phosphorylation and nuclear translocation of STAT6 in human bronchial epithelial cells. BEAS-2B (A) and NCI-H292 (B) cells were treated with wogonin and stimulated with IL-4 for 2 or 5 min. Phospho-STAT6, phospho-JAK1 and β-actin were assessed by Western blotting analysis. (C) BEAS-2B cells were treated with wogonin and stimulated with IL-4 for 30 min. Nuclear and cytosolic proteins were extracted from cells. STAT6 was detected by Western blotting analysis using anti-phospho-STAT6 and anti-STAT6 antibodies. (D) Culture conditions were identical to those in (C). Cells were fixed in 4% PFA, and then stained with anti-STAT6 antibody followed by Alexa Fluor goat anti-rabbit IgG. The data are representative of at least three experiments.