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. 2015 Jul 30;57(2):156–163. doi: 10.3164/jcbn.15-43

Fig. 3.

Fig. 3

BS enhances hyaluronan (HA) content via inhibition of HYAL2 in human dermal fibroblasts (HDF). (A) BS enhances HA content in HDFs. HDF cells were starved in serum-free media and treated with BS at the indicated concentrations for 48 h. HA concentrations were determined by ELISA as described in Materials and Methods. (B), (C), (D), (E) and (F) effects of BS on mRNA expression of an HA-regulatory enzyme in HDF cells. HDF cells were starved in serum-free media and treated with BS at the indicated concentrations for 24 h. mRNA levels of HAS1 (B), HAS2 (C), HAS3, HYAL1 (E) and HYAL2 (F) were analyzed by real-time RT-PCR using specific primers as described in Materials and Methods. (G) HA reduces HYAL2 protein expression in HDF cells. HDF cells were starved in serum-free media and treated with BS at the indicated concentrations for 48 h. HYAL2 and β-actin expression were determined by Western blot analysis as described in Materials and Methods. Data represent mean values ± SD. *p<0.05 vs the non-treated control.