Fig. 5.

BS enhances HA expression via recovery of HAS2 protein in UVB-treated HaCaT cells. (A) BS upregulates HA content in UVB-treated HaCaT cells. After 1 h of BS treatment, cells were irradiated with 0.01 J/cm² UVB. Media was collected after 48 h. HA concentrations were determined by ELISA as described in Materials and Methods. (B), (C), (D), (E) and (F) effects of BS treatment on mRNA expression of an HA regulatory enzyme in UVB-treated HaCaT cells. mRNA was harvested after 48 h. mRNA levels of HAS1 (B), HAS2 (C), HAS3, HYAL1 (E) and HYAL2 (F) were analyzed by real-time RT-PCR using specific primers as described in Materials and Methods. (G) HA reduces HYAL2 protein expression in HaCaT cells. HaCaT cells were starved in serum-free media and treated with BS at the indicated concentrations for 48 h. HAS2 and β-actin expression were determined by Western blot as described in Materials and Methods. Data represent mean values ± SD. *p<0.01 vs the UVB-treated control; ^#p<0.01 vs the non-treated control.