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. 2015 Aug;53(2):235–245. doi: 10.1165/rcmb.2014-0300OC

Figure 1.

Figure 1.

Expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) in blood eosinophils. (A) Real-time PCR exhibiting expression of NOX2, dual oxidase (DUOX) 1, and DUOX2 in blood eosinophils with low levels of NOX1, NOX3, and NOX5. No expression of NOX4 was detected. Transforming growth factor (TGF)-β1 did not have significant effect on the expression levels of the NADPH oxidase isoforms (n = 4). (B) Decreased NOX2 expression with eotaxin-1 in a time-dependent manner (n = 4). (C) The effect of IL-5, IL-13, IL-25, and IL-3 on the expression of NOX2 (n = 5). (D) A high level of NOX2 messenger RNA (mRNA) transcripts was seen in the eosinophils of subjects with moderate to severe asthma (n = 7) compared with subjects with mild to moderate asthma (n = 6) or healthy subjects (n = 6). There was no significant difference between eosinophils from healthy subjects and from subjects with mild to moderate asthma. *P < 0.05, ***P < 0.001, and ****P < 0.0001. GAPDH, glyceraldehyde phosphate dehydrogenase.