Chemotaxis of blood eosinophils. (A) TGF-β1 and TGF-β2 increased the migration of eosinophils that was blocked by the CLC3 inhibitor NPPB (n = 3). **P < 0.01. (B) Eotaxin-1 and eotaxin-3 increased the migration of blood eosinophils that was blocked by CCR3 and CLC3 inhibitors SB328437 and NPPB, respectively (n = 3). ***P < 0.001 and ****P < 0.0001. (C) The effect of phloretin, an inhibitor of CLC3/PKC, on TGF-β1–, TGF-β2–, eotaxin-1–, and eotaxin-3–induced chemotaxis of eosinophils (n = 4). **P < 0.01 and ****P < 0.0001. HPF, high-power field.