Table 2.

Primer Pairs Used in Quantitative PCR Analysis for Nicotinamide Adenine Dinucleotide Phosphate Oxidases

Primer	Accession (NCBI)	Sequence (5′–3′)	Product Size (bp)	Temperature (°C)
NOX1-F	NM_007052.4	TGCGCTGGACAAATGTTCCATTCC	192	60.3
NOX1-R	NM_007052.4	TCAGGAAGGCATCCACAAACAGGA	192	60.5
NOX2-F	NM_000397.3	AGACTTTGTATGGACGGCCCAACT	103	60.4
NOX2-R	NM_000397.3	AAGGCTTCAGGTCCACAGAGGAAA	103	60.2
NOX3-F	NM_015718.2	ACCGTGGAGGAGGCAATTAGACAA	109	60.2
NOX3-R	NM_015718.2	TTCCAGGTTGAAGAAATGCGCCAC	109	60.3
NOX4-F	NM_001143836.1	AGCAGAGCCTCAGCATCTGTTCTT	115	60.4
NOX4-R	NM_001143836.1	TGGTTCTCCTGCTTGGAACCTTCT	115	60.2
NOX5-F	NM_001184779.1	TGCTGCTCCTCCTCATGTTCATCT	135	60.0
NOX5-R	NM_001184779.1	TCCAGAAGTTGGGCCCATGAAAGA	135	60.5
DUOX1-F	NM_017434.3	AACAATTTGTGCGGCTACGGGATG	109	60.3
DUOX1-R	NM_017434.3	TCCTGCAGGGTGGTATTTCGGATT	109	60.3
DUOX2-F	NM_014080.4	AGTACAAGCGCTTCGTGGAGAACT	85	60.3
DUOX2-R	NM_014080.4	TCTGCAAACACGCCAACACAGATG	85	60.3
GAPDH-F	NM_002046.3	TCGACAGTCAGCCGCATCTTCTTT	94	60.6
GAPDH-R	NM_002046.3	ACCAAATCCGTTGACTCCGACCTT	94	60.5
18S-F	NR_003286.2	TCAACTTTCGATGGTAGTCGCCGT	108	60.3
18S-R	NR_003286.2	TCCTTGGATGTGGTAGCCGTTTCT	108	60.2

Definition of abbreviations: bp, base pair; NCBI, National Center for Biotechnology Information.