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. 2015 Sep;53(3):391–399. doi: 10.1165/rcmb.2014-0150OC

Figure 1.

Figure 1.

Type I collagen expression is high and microRNA (miR)-29c is low in idiopathic pulmonary fibrosis (IPF) fibroblasts. (A) Primary IPF (n = 6) and control human lung fibroblasts (n = 4) were seeded on polymerized type I collagen matrices for 4 hours. Left panel: Type I collagen protein expression was quantified by Western blot analysis. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is shown as a loading control. Right panel: miR-29c levels were quantified by quantitative RT-PCR (qRT-PCR) and are shown as the ratio of miR-29 to U6-2 small nuclear RNA (RNU) expression. (B) IPF fibroblasts (n = 3) were plated on tissue culture plastic (TC) or polymerized type I collagen matrices (PC). Left panel: Type I collagen expression was quantified by Western analysis. GAPDH is shown as a loading control. Right panel: miR-29c levels were quantified by qRT-PCR and are shown as the ratio of miR-29 to RNU expression.