Figure 6. mGluR and mAChRs contribute minimally to wave-induced MC calcium transients at P9.

(A) Schematic of mGluR-mediated pathway for increases in intracellular calcium in Müller glial cell. (B) XY plane of the IPL shows Cal-520 loading from an IP₃R2-KO P9 retina. The whole field of view was divided in 16 ROI represented by the white rectangles. (C) Example traces of spontaneous MC calcium signals (ΔF/F) in IP₃R2-KO retinas. (D) Summary of the effects of genetic and pharmacological manipulations of GPCR signaling in MCs using IP₃R2-KO mice, mAChR antagonist (50 µM atropine), and mGluR antagonists (8 μM LY341495, 100 μM MCPG, 100 μM MTEP).

DOI: http://dx.doi.org/10.7554/eLife.09590.017