Figure 5. The number of HC ribbon synapses is unchanged in cochleae with reduced Nrp2.
(A–D) Visualization of ribbon synapses (anti-Ribeye in green; white arrows) on IHCs and OHCs at P21. Nuclei are labeled with DAPI (blue). Overall no differences in ribbon synapse numbers were observed between genotypes. (E) Quantification of ribbon bodies per IHCs and OHCs in P8 and P21 cochleae. All differences were not significant (ns). n = 6 cochleae per genotype per age. (F) Percentage of labeled type II-like fibers (defined as SGNs that had crossed into the OHC region and turned toward the base) in each genotype at P21. n = 6 WT; 6 het. Although the data trended toward more fibers in the Nrp2+/− cochleae, the difference compared to the control group was not significant (ns). Error bars, sem. Scale bar, 8 μm.