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. 2015 Sep 11;5:13951. doi: 10.1038/srep13951

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Venn diagram of the identified proteins. 102 of the identified proteins were found in all developmental stages. Eight proteins were only present at E14, three proteins were unique to E16, and 30 proteins were only identified in P1. (B) Validation of proteomics data. I: Evaluation of the spot intensity of protein found to be involved in RA-pathway. The protein expression is regulated in the course of kidney development. II: Western blot analysis of some of the proteins, which are involved in the RA-pathway. Blots were performed with kidney extracts from E16 to P7 (seven days old pups) confirming the proteomics data. The full-length blotting images are presented in Supplemental Fig. 7.