Figure 3. Classification of the identified proteins according to biological process.

(A) Pie chart of the biological processes (GO annotations) in which the identified proteins are involved. Most of the proteins are involved in metabolic and cellular processes. A large part of the identified proteins were involved in developmental processes. (B) Pie charts of the classification of the identified proteins according to their biological functions. Proteins involved in cell division were identified at E14; these proteins were down-regulated in the course of development, whereas iron binding proteins were more prominent at P1. The other categories, redox-active center, protease, RNA-binding, mitochondrion inner membrane, signal, ion transport, and transcription were expressed in the different stages of kidney development. (C) Pie charts of the classification of the molecular function of the identified proteins. The classification revealed embryonic stage dependent expression of proteins involved in different pathways. Proteins involved in the category of transcription regulation decreased during the kidney development, other categories, like iron ion binding and carbohydrate binding were expressed in late stage of nephrogenesis. (D) Interaction map of proteins involved in transcription regulation. Strong interaction was observed between Trim28, Cbx1, Cbx3 and Cbx5.