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. 2015 Sep 11;5:13951. doi: 10.1038/srep13951

Figure 5. Staining of heterochromatin protein 1.

Figure 5

(A) Immunofluorescence co-staining of Cbx1 (red) and DBA lectin (green) in the cultured kidneys and enlarged section of the Cbx1 staining in black and white. Cbx1 is expressed in the metanephric mesenchyme and at the tips of the ureteric bud Western blot analysis showed a decreasing expression of Cbx1 during the development. (B) Immunofluorescence staining of Cbx3 (red) and ureteric bud (green) in the cultured kidneys and enlarged section of the Cbx3 staining in black and white. Cbx3 is mainly expressed in the renal vesicles, and in the comma- and s-shaped bodies, but was also present in the ureteric bud. Western Blot analysis showed high expression of Cbx3 until P1. (C) Immunofluorescence staining of Cbx5 (red) and lectin (green) in the cultured kidneys and enlarged section of the Cbx5 staining in black and white. Cbx5 showed expression in the vesicles, comma- and s-shaped bodies, and metanephric mesenchyme, whereas less expression of the protein was observed in the uretic bud. Western blot analysis showed high expression of Cbx5 until E19. (D) Immunofluorescence staining of Trim28 (red) and lectin (green) in the cultured kidneys and an enlarged section of the Trim28 staining in black and white. Trim28 was expressed in the ureteric bud and metanephric mesenchyme as well. Trim28 expression decreases significantly in the course of kidney development, and was highly expressed in the cap mesenchyme surrounding each UB tip and in the interaction interface between the metanephric mesenchyme and ureteric bud tips. Western blot analysis showed high expression of Trim28 in early stages. Yellow arrows show the cap mesenchyme, green arrows show the ureteric tip and the blue arrow shows the renal vesicle. The Western blot quantification is presented as a bar chart with error bars. Each bar represents the intensity means ± s.d. of blots from 3 independent experiments. Significant differences: *p < 0.05, **p < 0.01, ***p < 0.001. P1: new born, P7: 7 days old rats, P14: 14 days old rats, (A) adult. The full-length blotting images are presented in Supplemental Fig. 8.