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. 2015 Jul;53(1):50–59. doi: 10.1165/rcmb.2014-0289OC

Figure 6.

Figure 6.

CC chemokine receptor (CCR) 2 deficiency attenuates lung macrophage FRβ expression and uptake of folate-PEG-Cy5 in mice treated with LPS. (A) Representative images showing folate-PEG-Cy5–derived chest fluorescence and (B) quantification of folate-PEG-Cy5–derived photon counts over the chest in wild-type (WT) and CCR2 knockout (KO) mice at 48 hours after intratracheal injection of LPS (3 μg/g body weight). Imaging was done at 1 hour after intratracheal injection of folate-PEG-Cy5 (500 nmol/kg). Results were normalized to background fluorescence in mice before injection of folate-PEG-Cy5. (C) Representative images of folate-PEG-Cy5–derived ex vivo lung fluorescence and (D) quantification of folate-PEG-Cy5–derived photon emission from lungs of WT and CCR2 KO mice at 48 hours after intratracheal injection of LPS (3 μg/g body weight). (E) mRNA expression of FRβ in total lung macrophages from WT and CCR2 KO mice at 48 hours after intratracheal LPS (mean ± SEM; n = 4–6 per group; *P < 0.01).