Figure 1.

Polymorphisms and promoter activity of the regulator of G-protein signaling (RGS) 2 gene in patients with asthma. (A) Diagram of the RGS2 gene and single-nucleotide polymorphisms (SNPs). (B) Association of RGS2 polymorphisms with asthma. Two common SNPs, SNP1 (A638G) and SNP2 (G395C), were genotyped in 40 nonasthmatic subjects and 40 patients with asthma. Data were analyzed using a χ² test, which gave a P value of 0.0073. (C) RGS2 gene polymorphisms repress promoter activity. Human airway smooth muscle (ASM) cells were transfected with pGL3-luciferase (Luc) plasmids driven by SV-40 or the RGS2 wild-type (WT) or mutant promoter corresponding to SNP1 and SNP2. Results are presented as fold increase compared with pGL3 containing a SV-40 promoter. Data are means ± SE of three independent experiments in triplicate done in two primary ASM cell lines. *P < 0.05 and **P < 0.01 between groups. NCBI, National Center for Biotechnology Information; UTR, untranslated region.